introduction and method
Overview of cell reprogramming through history include key findings, recent progress, and potential of using reprogrammed cells for tissue transplantation
Use of fibroblast x hepatoma hybrids to identify key regulatory genes in fibroblast identity (including the genes you are testing).
Include a summary of results from the 2010 manuscript from my lab (attached), with number of candidate genes identified
B. Reprogramming of cells by targeting candidate gene expression (our lab results)
Include summary of Ray manuscript (attached)
Include summary of Huda Thesis (reprogramming of hepatoma by Snai2)
Include summary of Jahan thesis (reprogramming of hepatoma cells by CREG1)
C. Neuronal cell development
Provide overview of what is known about what drives neuronal cell development during embryology
Provide short summary of neurological diseases
D. Reprogramming of neuronal cells
Has anyone tried to reprogram neuronal cells? If so , what were they able to accomplish
Describe that you are asking whether overexpression of key genes that have been found to reprogram hepatoma cells can also reprogram neuronal cells.
method the same Huda’s thesis
